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Title: A study on the production and accumulation of betalains in cultured cells of Beta vulgaris
Author: Kalkatawi, Samia Jamal
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1997
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The aim of this project was to study the accumulation of betalains in suspension cultures of Beta vulgaris. Betalains were found to accumulate in suspension cultures of Beta vulgaris in association with growth. The onset of pigment synthesis occurred prior to, or immediately after, the start of cell division. The production of betalains could be influenced by the conditions of culture differently from growth. Varying the concentration of inorganic nitrogen (i-N) or inorganic phosphorus (i-P); above or below that in B5 medium, did not increase either growth or pigment production. 3% sucrose was shown to be optimum for betalain accumulation while the fresh weight of culture was not affected. Lowering the concentration of 2,4-dichlorophenoxyacetic acid (2,4D) favoured betalain production. The highest pigment content per culture was achieved at 0.006 mg.1-1 2,4D at which concentration the culture growth was not significantly affected. Altering the concentration of kinetin in the medium did not increase the production of betalains. Administration of either tyrosine or dihydroxyphenylalanine (DOPA) did not significantly affect either growth or pigment production. A single cell plating techniques was used successfully to isolate a number of cell lines (violet, orange, yellow and white) with different qualitative and quantitative characteristics with regard to betacyanin and betaxanthin content. HPLC analysis of the betalains produced by the selected cell lines and the mother culture, revealed that betanin and miraxanthin V were the main betalains produced by the mother culture and the derived pigmented cell lines. These cell lines underwent changes in pigment production during a period of non-selective subculturing (15 months). The production of betalains in these cell lines was investigated in relation to cellular heterogeneity and the study showed that the cellular population of the cell lines contained a limited proportion of cells that accumulated the pigment.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available