Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.653195
Title: Stromal cell function in non-pregnant endometrium and decidua of early pregnancy
Author: Kane, Nicole M.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2007
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Abstract:
The aims of this research project have been to investigate the role of TGFβ1 in mediating factors involved in decidualization, with particular regard to expression of nuclear progesterone receptor (PR), the Wnt antagonist, Dickkopf-1 (DKK) and markers of decidualisation in order to identify a potential role for TGFβ1 in decidualisation and menstruation, and to further characterise the uNK cell to extrapolate how uNK cells may interact with other uterine hormones and ESCs during the secretory phase and early pregnancy. In primary human ESC cultures, both non-decidualised and decidualised in vitro PR and DKK expression levels were downregulated after TGFβ1 treatment in both non-decidualised and decidualised cells but TGFβ1 was without effect on progesterone binding to the progesterone response element (PRE). In addition TGFβ1 treatment of in vitro decidualised ESCs and stromal cells from 1st trimester decidua inhibited production of classical decidualisation markers. SMAD4 knockdown employing siRNA techniques revealed that TGFβ1 was not acting via the SMAD signalling pathway and may be acting via alternative pathways e.g. Wnt, MAP kinase and JNK signalling. Primary cultures of human uNK cells have been used for studying the possibility that LH and hCG may be the hormones responsible for the influx and proliferation of uNK cells. LH/hCG receptors were not expressed on the uNK cells, however, mannose receptors, which have been proposed to bind hCG, were localised to uNK cells. Furthermore, when uNK cells were treated with hCG, colocalisation with hCG and mannose receptors was observed. Further investigation into the interactions between the mannose receptor and LH/hCG would be important in clarifying the mechanisms controlling uNK influx and proliferation. The interactions between ESCs and uNK cells need to be clarified further to assess the roles of uNK cells in reproductive processes. This work has thus revealed a potential novel mechanism for the influx and recruitment of uNK cells mediated by the mannose receptor.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.653195  DOI: Not available
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