Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.652837
Title: Studies on some determinants of virulence in Alcelaphine herpesvirus 1
Author: Ireri, R. E.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1999
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Abstract:
Malignant catarrhal fever (MCF) is a fatal lymphoproliferative of Artiodactyla. The disease is caused by infection of susceptible hosts with one of two, gammaherpesviruses, Alcelphine herpesvirus-1 (AHV-1) or Ovine herpesvirus-2 (OHV-2). On primary isolation AHV-1 infectivity is cell-associated and the virus can induce MCF following susceptible into susceptible hosts. Cell free virus which is pathogenic for cattle is observed following low serial passage of the virus in cell cultures. After further serial passage cell free virus is observed, but this virus cannot produce disease experimentally. AHV-1 genomic rearrangement occur during the transition from virulence to attenuation. Two genes, encoding putative protein 5 (P-5) and protein 1 (P-1), are truncated during this rearrangement. Sequence encoding each of these proteins was cloned and the proteins expressed in vitro. Rabbits, a laboratory model for AHV-1, were successfully immunised with these proteins. Neither of these proteins however induced a protective immune response. Although both proteins are expressed in vitro transcripts for these proteins could not be detected in vivo in animals reacting with MCF. During the course of this study, the complete sequence of the AHV-1 genome was published. P-1 and P-5 were shown to form part of ORF 50 and A7 respectively. These ORFs were therefore reassessed to determine their positions in the attenuated and virulent virus. New isolates were obtained from cattle showing clinical MCF and also from wildebeest. Viral DNA derived from new isolates has shown that ORF 50 and ORF A7 are conserved between isolates and the virulent laboratory adapted isolate. The results presented here show that a block of sequence, which includes sequence for ORF 50 and its promoter are translocated from a position in the middle of the unique NDA to a terminal position where it is inverted with respect to its transcriptional orientation in the virulent virus. The ORF is transcriptionally silent in the attenuated virus and the results presented here show that this is due to the inactivity of the truncated ORF 50 promoter in the attenuated virus.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.652837  DOI: Not available
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