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Title: Studies on hydrogen ion secretion by the gallbladder epithelium
Author: Plevris, Ioannis (John)
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1995
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In this thesis the in vitro ability of gallbladder epithelium to secrete hydrogen ions, the mechanism and regulation of the acidification process and its pathophysiological consequences to calcium salt solubility in bile, were studied. The human gallbladder epithelium was capable of secreting hydrogen ions, through a sodium/hydrogen apical exchange system (antiport) and its acidification ability was found reduced with inflammation. The normal bovine gallbladder epithelium was also capable of secreting hydrogen ions through a sodium/hydrogen apical exchange system that was ouabain and amiloride sensitive. Acidification was histamine dependent because it was stimulated by histamine and inhibited by H1 and H2 antagonists. Cholecystokinin augmented acidification but acetazolamide (carbonic anhydrase inhibitor) did not have any effect on acidification. Normal bovine gallbladder bile had striking similarities with human and bile acidification significantly improved calcium carbonate solubility but had no effect on calcium phosphate solubility. In addition the anatomical similarities of human and bovine gallbladders were confirmed by pathology studies. Primary gallbladder epithelial cell short-term cultures were developed to enable a closer study of the biological properties of the gallbladder epithelial cell. Acid production as well as the modulator effect of amiloride and histamine was visualised in gallbladder epithelial cell suspensions with the use of the fluorescent dye, acridine orange. In this thesis it was demonstrated that the gallbladder epithelium secretes hydrogen ions into the bile through a sodium-hydrogen apical antiport system and this may serve as a protective mechanism against calcium carbonate precipitation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available