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Title: Comprehensive analysis of human CpG island methylation
Author: Illingworth, Robert S.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2008
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We devised a preparative chromatographic tool for the enrichment of CGI sequences based on the empirical criterion of clustered nonmethylated CpGs. Characterisation of a novel somatic CGI set derived from human blood DNA identified a population of islands which were missed by commonly applied bioinformatics criterion. The identified CGIs were enriched in gene rich portions of the genome, and preferentially associated with chromosome ends proximal to the telomeres. Despite association with gene promoters, approximately fifty percent of CGIs were found to be either intra- or intergenic. An arrayed set, comprising ~17,000 unique sequences, allowed the investigation of the methylated CGI component of human blood, brain, muscle and spleen. This identified 6-8% of normally methylated CGIs across the 22 human autosomes. These hypermethylated islands were enriched in regions distal to annotated promoters and preferentially associated with developmental gene loci. The reagents and observations discussed in this thesis allow detailed analysis of the abundance and distribution of methylated CGIs in ‘normal’ human somatic cells.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available