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Title: Bovine immune responses to Fasciola hepatica during the early stages of infection
Author: Hoyle, D. V.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2000
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This study was designed to investigate the effect that pre-exposure of cattle to 1-2 and 5-6 day old flukes had upon subsequent challenge infection, when compared to naive and chronically infected animals. Infection parameters, together with peripheral and local cellular and antibody responses to various F. hepatica protein preparations were investigated. A primary exposure to 5-6 day old flukes, terminated by triclabendazole treatment, was found to reduce the degree of liver damage and eosinophilia experienced after a challenge infection. Lower levels of the enzymes gamma-glutamyl transferase and glutamate dehydrogenase were detected in sera, compared to those in naive animals (P < 0.05). Eosinophilia was also reduced (P < 0.01), as was egg out-put in the faeces during the early pre-patent period (P < 0.05). Calves that were pre-exposed to 1-2 day old flukes showed no significant differences in the levels of these parameters, when compared to their naive counterparts. Sera taken prior to the secondary challenge from pre-exposed animals and those receiving an unterminated primary infection recognised a variety of proteins in Western blots of whole somatic adult antigen (WFA) and excretory-secretory (ES) preparations. The IgG1 antibody response to protein bands of 96-82, 76-68 and 60-52 kDa predominated. After secondary challenge the response of the chronically infected animals to these protein species was reduced and extremely strong recognition of bands in the region 30-28 kDa was noted. Pre-exposed animals maintained the response to the higher weight bands, showing a similar, but initially stronger recognition pattern, to that of naive challenged animals. The lower weight protein bands were not detected in these groups until much later. The IgG1/IgG2 isotype antibody response to purified cathepsin and haem-containing high molecular weight fractions were also examined by ELISA. A monophasic, IgG1 response was seen to the cathepsin fraction, which occurred later during the infection process and was not seen prior to secondary challenge in any of the four groups. A mixed IgG1/IgG2 antibody response to the haem fraction was seen within 14 days of primary infection.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available