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Title: The isolation, culture and characterization of Gram-negative non-sporing anaerobic bacilli, with special reference to the occurrence of Bacteroides species in the human mouth
Author: Holbrook, W. P.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1976
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Abstract:
A scheme for the identification of Gram-negative, nonsporing, anaerobic bacilli is presented. This is based on the results obtained with strains obtained, from culture collections, strains referred by other interested workers, isolates from clinical specimens, isolates from normal human faeces, and isolates from subgingival dental plaque. The tests employed were conventional bacteriological tests adapted for use with these organisms. They included: tests of tolerance to certain dyes and bile salts; resistance patterns obtained with antibiotic disks; and a number of biochemical tests including carbohydrate fermentation tests. A clear distinction could be made between the various reference strains - Bacteroides fragilis, B. melaninogenicus, B. oralis, Fusobacterium necrophorum and F. polymorphum being the main species recognised. Most B. fragilis and B. melaninogenicus strains could be placed into one of the recognised subspecies, but some organisms gave patterns of results intermediate between those of recognised subspecies. Within the particular species, strains of B. fragilis and B. melaninogenicus seem to form a continuum of variants with clusters of strains in the designated subspecies. A number of strains of B. melaninogeni cus were isolated that would only grow in mixed culture. In efforts to solve this problem several improved media were tested in the laboratory and some were evaluated in the clinical situation. The transport of specimens in Moller's VMG II transport medium allowed recovery of B. melaninogenicus strains comparable with that obtained by the direct plating of the sample. Incorporation of kanamycin and vancomycin into the plating medium enhanced recovery of B. melaninogenicus and permitted growth of the other recognised species of oral, Gram-negative, anaerobic bacilli. The strains of B. melaninogenicus ss. melaninogenicus and B. oralis isolated in this survey were characterised by the tests developed in the first part of the study. The taxonomic significance of the results is discussed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.652503  DOI: Not available
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