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Title: Mechanisms underlying postnatal development of primary somatosensory cortex
Author: Hillen, M. J. P.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2006
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As a first step in examining a role for Wnts in barrel formation, the gene expression patterns of members of the Wnt, Frizzleds and secreted Frizzled related protein (sFRPs) families during barrel cortex development were measured using degenerate primer RT-PCR, quantitative real-time PCR and in-situ hybridization. Wnts 2b, 3, 4, 5a, 7a, 7b, 9a, 11, 16 were found in the barrel cortex, suggesting that these members of the Wnt family may play a role in barrel development. In order to determine whether Wnts, members of the Wnt signalling machinery and MAGUKs associated with Wnt signalling are essential for barrel formation, the barrel phenotype of all available postnatally viable Wnt gene knockouts, Wnt2b-/-, Wnt8b-/-, Wnt signalling component knockouts Dvl-/-, MAGUK knockouts SAP-102-/-, PSD-95-/- and double knockouts of Wnt7a-/-Dvl-/- and of SAP-102-/--PSD-95-/- were examined. Barrels appeared normal in all mutants, apart from compound SAP-102+/--PSD-95+/-and SAP-102-/--PSD-95+/- and SAP-102-/-PSD-95+/- mice, which exhibited poorer barrel segregation compared to wild type. In order to achieve a detailed understanding of the mechanisms by which neuronal activity regulates barrel development, we need a detailed understanding of the intracellular pathways activated by NMDA receptor during development. In searching the literature, data concerning the developmental expression patterns of NRC components can be difficult to locate, as the prevailing database tools used either search only title, author and keyword abstract text (NCBl PubMed) potentially missing desired information, or as in the unique case of Google Scholar, search the full text of electronically published papers, but yield overwhelming numbers of results in the process.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available