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Title: Synthetic approaches to discontinuous epitope mapping of HIV-I
Author: Heslop, Ian
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1997
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The synthesis of IH1, a peptide designed to mimic a discontinuous epitope on HIV-I gp120, is reported. The aspartimide rearrangement inherent in this sequence, and in the peptide GC1, has been studied and reduced to low levels. The syntheses of variants of peptide GC1, containing differing number of residues found to be important for CD4 binding, have also been achieved. Thus peptides containing one, two, three and four residues necessary for high affinity binding have been synthesised. In addition a peptide has been synthesised which incorporates a synthetic β turn moiety other than the Cys-Val-Cys bridge present in GC1. Polyclonal sera raised to these peptides and their CD4 binding properties have been investigated. IH1, the peptide containing four residues responsible for high affinity binding to CD4, has also been shown to interact with receptors on the surface of CD4- cells. This non-CD4 recognition has been investigated utilising a photolabelled chemokine, MIP-1α. Results indicate that this binding involves interaction with CC-CKR5, a MIP-1α binding site thought to be involved in HIV-cell fusion.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available