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Title: Comparison of virulent and non-virulent Alcelaphine herpesvirus-1 derivatives
Author: Handley, J. A.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1994
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Malignant catarrhal fever (MCF) is a fatal lymphoproliferative disease affecting ruminants. The disease is caused by infection of susceptible hosts with one of two gammaherpesviruses, Alcelaphine Herpesvirus-1 (AHV-1) or Ovine Herpesvirus-2 (OHV-2). On isolation AHV-1 infectivity is cell-associated and will induce MCF on inoculation into experimental animals. After serial passage cell-free infective virus is observed, but this virus cannot produce MCF experimentally. The aim of this project was to characterise the genomic alterations which occurred in one isolate, C500, associated with this altered pathogenicity. Viral DNA of virulent (PP) and cell-free attenuated (CFA) C500 derivatives was compared by restriction endonuclease profiling. Variability was observed using Sma I, as a 5kbp fragment was present in PP DNA but not CFA DNA. Conversely a 3.8kbp fragment was present in CFA DNA but absent from PP DNA. Homology was observed between these Sma I fragments. The Sma I 3.8kbp fragment was cloned (ATT-1), as were two Hind III equivalents of the PP 5kbp Sma I fragment (VIR-1 and VIR-2). These clones were mapped for several restriction endonucleases, subcloned and sequenced. The location of the C500 clones was assessed by Southern blotting and PCR. The structure of the C500 genome consisted of a central unique region of approximately 130kbp, flanked on either side by multiple copies of a 1-5-kp repeat unit, resulting in an overall genome size of 160kbp. The ATT-1 clone was found to be present twice in the CFA genome, located at both ends of the unique region, close to the terminal repeats, with both copies orientated in the same direction. The location of the VIR-1 and VIR-2 clones in the PP genome was not fully determined during this study, however, the results obtained suggested that VIR-1 and VIR-2 clones were located approximately 1.4kbp apart, at one end of the unique region, close to the terminal repeats.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available