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Title: Physiological and anatomical studies in seed coat regulation of water uptake in soybeans (Glycine max L. Merril)
Author: Hahalis, Demosthenis
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1997
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In this study, imbibition damage due to rapid water uptake was well documented in a wide range of soybean genotypes. In addition a line (VLS-1) was identified that possessed a delayed-permeability seed coat characteristic that offered protection against imbibition damage. This characteristic was likely to be due to a lack of pits in the abaxial region of the seed. In contrast, genotypes with a high proportion of deep and wide open pits in the abaxial region of the seed offered minimal protection against imbibition damage. Coating seeds (24 mg per seed) with a polymer containing vinyl acetate, vinyl chloride, ethylene and acrylate regulated the rate of water uptake, and offered protection against imbibition damage. Seedling emergence from polymer coated seeds was also improved. Deposits and pits occurred in the surface of the seed coat in most genotypes. Deposits were shown to be composed of hydrophilic polysaccharide material, since staining with calcofluor was observed. Water permeability mapping indicated that pits were the sites of the initial water penetration. However, in hard seeds, pits appeared to function in a different way to soft seeds and this is fully discussed within the thesis. Prolonged methanol pre-treatments were highly effective in increasing the water uptake when seeds were imbibed immediately after the pre-treatments. However, drying of seeds after the organic solvent pre-treatments restored permeability to water to untreated control levels. Results from the absorption spectrum of the methanol and chloroform supernatants, indicated that the effect of the pre-treatments were not due to the extraction of phenolic material from the seed coat. In hard seeds, the location of the water impermeability barrier was near the outermost part of the palisade cell layer. The nature of the barrier was not identified by comparative anatomical and histochemical studies between hard and soft seeds.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available