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Title: Induction of PAL during elicitation of Capsicum frutescens Mill. cell cultures
Author: Groskurt, Ernst Eugen
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1992
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This project was an investigation of the regulation of PAL induction, following elicitation of cell cultures ofCapsicum frutescens, and its effects on phenylpropanoid metabolism. It was shown that the response of PAL specific activity to elicitation increased throughout the culture cycle and consequently, stationary-phase cultures were used throughout the investigation. PAL activity in Capsicum cultures increased transiently after elicitation. Detailed investigation of the initial stages of elicitation showed that PAL activity was induced 40 min after the onset of the elicitor treatment and reached a maximum after ca. 8 h. Capsicum PAL protein sub-units were identified and the molecular size was estimated to be 77 kD, the same size as PAL sub-units isolated from parsley leaves. In vivo labelling techniques showed that the transient induction of PAL specific activity following elicitation resulted from a combination of the general induction of protein synthesis and a specific induction of PAL protein synthesis. Additionally, the synthesis of a 32 kD protein, probably a chitinase precursor, was transiently induced with a lag phase of 4-8 h. A 70 kD polypeptide, possibly a degradation product of PAL sub-units, was recognised by anti-(parsley PAL) serum in samples from elicited and control cultures ofCapsicum frutescens but not in Petroselinum samples. PAL mRNA was identified and its molecular size was estimated to be 2.6 kb. Increased PAL mRNA steady state levels were detected following dilution and elicitation. The transient increase in PAL mRNA levels during elicitation followed the same pattern as PAL protein accumulation, thus indicating that the induction of PAL is regulated at the transcriptional level. Elicitation of Capsicum cultures increased PAL mRNA levels transiently resulting in de novo synthesis of PAL protein and, thus, increased PAL activity.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available