Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.651839
Title: Pax6 gene in the development of the eyes, nose and brain
Author: Grindley, Justin C.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1995
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Abstract:
Small eye (Sey) mouse embryos, homozygous for mutations in the Pax6 gene, have no eyes, no nasal cavities and no olfactory bulbs (Hogan et al, 1986; Hill et al, 1991). Investigating the basis of this striking phenotype could potentially provide valuable insights into the normal processes involved in the development of the eye, nose and brain. Pax6 encodes a transcription factor, with two DNA-binding motifs, a paired domain and a paired-type homeodomain (Walther and Gruss, 1991). The work described in this thesis is an investigation into the potential roles of this regulatory gene during normal development. Information obtained from studying the pattern of Pax6 mRNA expression in wild-type embryos, and the developmental defects of Sey/Sey animals, was combined to identify processes that may involve PAX6. PAX6 is essential for the formation of lens placodes from surface ectoderm. In normal development, early Pax-6 mRNA expression in a broad domain of surface ectoderm is downregulated, but expression is specifically maintained in the developing lens placode. Thus, phenotype and expression together suggest a role for PAX6 in lens determination. In surface ectoderm in the eye region, PAX6 function is also required for the maintenance of Pax6 transcription. Like the lens, the nasal cavities develop from ectodermal placodes that normally express Pax6 mRNA, fail to form in Sey/Sey mice and show PAX6 dependent Pax6 mRNA regulation. Analysis of patterns of programmed cell death and absence of nasal region expression from an Msx1 transgene in Sey/Sey embryos, suggest a requirement for PAX6 in the transition from presumptive nasal ectoderm to placode, and that Msx1, or genes regulating it, are possible targets for PAX6.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.651839  DOI: Not available
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