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Title: Isolation of adaptive quantitative trait loci in Antirrhinum
Author: Greenshields, David
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2007
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Understanding the genetic basis of quantitative traits represents a huge goal in modern molecular and evolutionary biology. Here, the natural genetics of the genus Antirrhinum, within which separate species can be successfully interbred, are used to investigate differences in a range of morphological characteristics. The two species used in the study, Antirrhinum majus and Antirrhinum molle, have become adapted to very diverse environments and consequently exhibit large variance in a wide range of traits. A large-scale FI mutant screen, from a cross between a transposon-active A. majus line and A. molle, isolated segregating mutations for flower size, flower colour, trichome density and branching in self-pollinated F2 populations. Amplified Fragment Length Polymorphism analysis of the F2 and the use of molecular maps have shown the mutations generally correspond to known Quantitative Trait Loci, and the roles of genes linked to these regions are discussed. The technique sheds some light on the molecular and evolutionary mechanisms underpinning diversity in Antirrhinum and has implications for the use of transposon-tagging in locating QTL in other plant systems.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available