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Title: Immunoliposomes in haematology
Author: Gray, Atherton
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1992
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The aim of this thesis was to use antibody-directed liposomes to enhance the targeting of 1. fluorescent compounds for the detection of cell surface antigens and 2. cytotoxic compounds for delivery to specific cell populations. This involved the selection and production of a suitable species of liposome-protein conjugate from among those described in the literature. The choice was a small unilamellar liposome conjugated either to antibody or protein A via a covalent linkage mediated by the heterobifunctional reagent SPDP. Anti-immunoglobulin antibody was a more universal ligand than either specific antibody or protein A. The conjugate was made fluorescent by the encapsulation of carboxyfluorescein (CF) in the liposome and the maximally fluorescent concentration was determined at 20 mM CF. The probe was stable on storage over many months and had low non-specific staining characteristics. Applied to lymphocytes it gave a signal enhancement in order of magnitude greater than the conventional fluorescent-antibody method as measured by flow cytometry. This permitted the demonstration of the interleukin-2 receptor on resting polyclonal T-lymphocytes hitherto undetectable by conventional fluorescence. This set the threshold of detectability at approximately 1000 antigen copies per cell.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available