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Title: Immunopathological studies in the ovine lung during the course of natural and experimental parainfluenza type 3 virus infection
Author: Gonzalez, C. M.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1996
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The pulmonary immunopathology of parainfluenza type 3(PIV-3) infection in sheep was investigated firstly by isolating the virus from field cases of sheep pneumonia, secondly by experimentally reproducing the disease with the isolated virus and finally by studying changes in lymphocytes subsets and alveolar macrophages, induced by PIV-3 in vivo and in vitro. Three ovine virus isolates(270-7, 390-10 and 430-7) were obtained and characterised, as PIV-3, according to virus morphology, under transmission electron microscopy(TEM); cytopathic effect(CPE); haemagglutination, of guinea pig erythrocytes; physicochemical properties; serological crossreactivity with antisera raised against PIV-3, isolated from different species; and reactivity with monoclonal antibodies anti PIV-3 structural proteins, crossreacting with human and bovine strains. The ability of the virus to induce respiratory disease was investigated by experimental inoculation of ovine PIV-3 isolate, 270-7, in colostrum deprived lambs. Clinical, pathological, bacteriological and virological studies were carried out on days 2,3,5 and 7 infection(p.i.). This PIV-3 ovine strain was able to induce clinical disease. Histopathological findings were interstitial pneumonia with hyperplasia of bronchiolar associated lymphoid tissue (BALT), degenerative bronchiolar epithelium with lymphocyte infiltration, areas of atelectasis and increased alveolar septa thickness due to proliferation of pneumocytes II, lymphocytes, macrophages and later to fibrosis. The average cell count per normal BALT was 1,307(range 1,205 to 1,480 cells) whereas in infected lungs on PID 7 the average count increased to 4,788 (range 4,390 to 4,930 cells). The large number of lymphocytes, particularly on days 5 and 7 p.i., combined with the minimal to moderate cytolysis of antigen bearing cells, suggests that PIV-3-induced pulmonary disease has an immunopathological component. PIV-3 particles were detected, by immunohistochemistry, more frequently in bronchiolar epithelium cells than in alveolar septal cells and macrophages by day 3 p.i., but the situation was opposite by day 7 p.i.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available