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Title: Characterisation of genomic clones encoding sex-specific transcripts in Drosophila melanogaster
Author: Gilman, Olivia Claire
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1991
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This Thesis describes the characterisation of five genomic clones from Drosophila melanogaster: λfs1, λfs3, λfs4, λfs6 and λfs7, which were isolated in a differential cDNA screen for genes encoding non-gonadal female-specific transcripts. Northern analysis was used to identify transcripts encoded by the genomic clones in adult males and females, and to investigate their spatial and temporal expression patterns. In addition, in situ hybridisation studies on whole tissue preparations were used for more accurate determination of transcript distribution, being particularly successful in ovarian tissue preparations. Clones λfs3, λfs4, λfs6 and λfs7 were found to contain DNA with an interesting pattern of cross-hybridisation. This is described in detail and possible explanations discussed. Finally, the justification for the isolation of these clones from a screen for genes with non-gonadal female-specific expression is considered.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available