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Title: Analytical and structural studies of Acacia polysaccharides
Author: Gill, M. C. L.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1978
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An analytical study of eleven different species of Acacia gum from the subseries Juliflorae of the series Phyllodineae showed them to be more proteinaceous, more acidic, and more viscous, with higher molecular weights but with lower proportions of rhamnose and arabinose, than the majority of the other species of Acacia gum studied so far. The results confirm the need for a reappraisal of the taxonomy of the subseries Juliflorae which has been suggested by botanists on morphological grounds. A structural study of the gum from Acacia auriculiformis involving hydrolysis, Smith-degradation and methylation studies revealed a 2-1, 3-linked galactose backbone with side-chains of P-1, 6-linked galactose. Glucuronic acid is present as end-groups, /--1, 6 -linked to galactose; 4-0-methylglucuronic acid is present as end-groups, -1, 4 linked to galactose. Arabinose is present in short, ,9-1, 3- linked chains, and rhamnose is present as end-group. Proteinaceous material was attached to the molecule by periodate-resistant linkages; the direct linkage of polysaccharide to protein in plant gums of the Acacia genus has been suspected for some time, but this is the first clear-cut experimental evidence in structural studies for this important feature. The use of carbon-13 nuclear magnetic resonance spectroscopy for the study of gum exudates was examined. Spectra were obtained for the gum from Acacia auriculiformis and its degradation products, despite their high molecular weight and chemical complexity. The spectra substantiated the findings of the structural study, and some assignments of characteristic resonances for some of the specific functional groupings.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available