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Title: Interactions amongst polycomb-group genes regulating flowering in Arabidopsis
Author: Chanvivattana, Yindee
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2002
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The recessive moe leaf (moe) mutation confers a similar phenotype to clf mutations. Molecular and genetic studies indicated that like CLF, MOE activity is required to repress floral homeotic gene expression suggesting that the wild-type MOE gene might define an additional Pc-G protein. This was confirmed by the isolation of MOE. Thus mapping of MOE showed that it is located to the region of a recently isolated Arabidopsis Pc-G member, the EMBRYONIC FLOWER2 (EMF2) gene which play an important role in repressing floral genes during vegetative development. Subsequent genetic analysis indicated that moe is allelic to emf2 mutations and cloning of EMF2 locus from moe mutants showed that the moe allele harbours a small deletion in the EMF2 sequence. It appeared that moe represents a novel weak allele of EMF2 and its phenotype was unlike that of other emf2 alleles described, and informative as to EMF2 function. In particular, genetic analysis using moe and weak clf alleles suggested that EMF2 has a close functional relationship with CLF. Reverse genetic approaches were used to determine the function of an Arabidopsis CLF homologue called CURLY LEAF LIFE-1 (CLK1). CLF and CLK1 show extensive sequence similarity suggesting that they have a common evolutionary origin. Investigation of mRNA localisation by in situ hybridisation indicated that CLF and CLK1 RNA expression patterns are very similar. In addition CLF and CLK1 proteins show common interactions in yeast two-hybrid assay. Together these findings suggested that CLF and CLK1 may show functional redundancy. Characterisation of transgenic lines expressing a dominant negative CLK1 construct, and of a line carrying a T-DNA insert in the CLK1 5’UTR, both provided strong support for this hypothesis. Thus reduced CLK1 activity had little or no effect in wild-type background, but strongly enhanced the clf phenotype.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available