Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.650926
Title: Oestrogens and consequences for the excurrent ducts in the male
Author: Fisher, Jane Susan
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2000
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Abstract:
The aim of the experimental work in this thesis was to identify the sites and potential roles of oestrogen in the development and function of the male reproductive tract, particularly within the excurrent ducts. The sites of oestrogen receptor-beta (ERb) immunoexpression were identified within the testis and epididymis of the marmoset monkey from perinatal to adult life and compared to those of oestrogen receptor-alpha (ER-a) and the androgen receptor (AR). One common site of expression, the efferent ducts, was focused on in the remainder of the studies. Neonatal rats were injected on alternate days between days 2 - 12 with DES at 3 doses (10, 1 and 0.1mg/injection) or with ethinyl oestradiol (EE; 10mg/injection) or with high doses of one or other weakly oestrogenic compounds (genistein, octylphenol, bisphenol A or parabens) or the agonist/antagonist Tamoxifen. To establish whether the effects of oestrogen treatment were direct or indirect (i.e. arising from LH/FSH secretion) all parameters investigated were also analysed in rats treated neonatally with a gonadotrophin-releasing hormone antagonist (GnRHa). It is concluded that physiological levels of oestrogen are required for normal testis growth and spermatogenesis, and that oestrogens are involved in maintaining normal fluid dynamics within the excurrent duct system. Oestrogens appear to play a role in the development of the efferent duct epithelium as both cell morphology and function were altered after exposure to oestrogens. The reduced fluid resorption suggests this function is at least in part modulated by oestrogen. Oestrogen exposure also influenced androgen action as the level of androgen receptor detected by immunocytochemistry after the neonatal oestrogen treatment was negligible or greatly reduced. More specific endpoints of oestrogen action have yet to be identified and research is required to allow a fuller understanding of the roles of oestrogen and whether exposure to environmental or dietary oestrogens pose any risk to human reproductive health.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.650926  DOI: Not available
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