Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.650836
Title: Regulation of gene expression in the immature Sertoli cell : a role for oestrogens?
Author: Fenelon, Miriam Claire
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2001
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
Abstract:
In the first part of the thesis changes in the temporal and spatial expression of Sertoli cell mRNA and proteins were investigated in the postnatal rat testis. Novel results regarding ERb expression during this time of development were reported. In addition patterns of Musashi-1, GATA-1 and GATA-4 Sertoli cell expression were described including new information demonstrating changes in Musashi-1 and GATA-1 protein localisation in the Sertoli cell during the transition from proliferating cells to mature differentiated Sertoli cells. Artificially elevated levels of oestrogen in the neonatal testis delayed Sertoli cell maturation and subsequently delayed expression of GATA-1 protein demonstrating the restriction of GATA-1 protein expression to non-proliferating Sertoli cells which has not previously been described. A primary rat Sertoli cell culture system was established in which Sertoli cells retained hormonal sensitivity consistent with the expression of androgen receptor and ERb proteins. New preliminary data describing upregulation of ERb protein levels by oestrogen in cultured rat Sertoli cells are reported. Steroid regulation of GATA-4 expression in cultured rat Sertoli cells was not detected in the present study at the level of either mRNA or protein. Problems obtained sufficient numbers of Sertoli cells in culture during this investigation was overcome by employing an immortalised mouse Sertoli cell line from the H-2Kb-tsA58 transgenic mouse and conformation of results obtained using primary cultures of rat Sertoli cells was carried out. In conclusion, the studies outlined in this thesis have described expression patterns of markers of Sertoli cell functional maturation and preliminary data regarding their steroid regulation. Such new data can be employed in future studies to further evaluate direct effects of oestrogen and oestrogen-like chemicals on Sertoli cell behaviour in the postnatal rat testis with a view to elucidating the mechanisms involved in modification of testicular function by oestrogens.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.650836  DOI: Not available
Share: