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Title: Bone morphogenetic proteins (BMPs) : new modulators of the follicle stimulating hormone (FSH) synthesis and release
Author: Faure, Marie-Odile
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2006
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To extend our knowledge on FSH synthesis regulation, we investigated the potential role of BMPs in the ewe pituitary. The two types of BMP receptors (BMPR-IA and BMPR-II) co-localised with gonadotrope cells and their ligands (BMP-2, BMP-4, BMP-7 and GDF-9) mRNAs were detected in the pituitary suggesting a paracrine action of BMPs.  BMPR-IB \was not detected on gonadotropes. Interestingly, in three breeds of ewes, BMP-4 as well as BMP-6, BMP-15 and GDF-9 inhibited specifically the FSH synthesis and release, but not the LH production from ewe pituitary cells. Moreover, BMP-4 antagonized the stimulatory effect of activin and amplified the inhibitory action of 17β-estadiol. To determine at which level BMP-4 was able to act on FSH differential secretion, we investigated the Smads signalling pathways. BMP-4 activated the phosphorylation of Smad-1/5 without affecting Smad-2 phosphorylation level triggered by activin. The blockade by inhibitory Smad, Smat7 showed the role of the Smad signalling in BKP-4 action. Blocking the activin signalling by follistatin suggested independence between activin and BMP-4 signalling pathways. In the mouse gonadotrope cell line (LβT2), BMPs and their receptors mRNAs were detected. In contrast to ewe, BMP-4 alone was not able to modulate the gonadotropin secretion. However, BMP-4 amplified the stimulatory effect of activin and GnRH on FSH secretion. Moreover, BMP-4 inhibited the stimulatory effect of activin and GnRH on LH production by inhibiting the expression of GnRH receptor. These differences of effect due to the species underlined the necessity of studying different models to enhance our understanding of reproductive function.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available