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Title: The host-parasite interaction, with particular reference to Trypanosoma evansi antigens
Author: El Hassan, El Awad Mohammed
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1995
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The objective of the present study was to examine the ways that the T.evansi components interact with the host by investigating the components of the parasite which acted as antigens during infection and to study the dynamics of some of these antigens during infection. Results from this study could help in identifying new diagnostic reagents, a better understanding of existing diagnostic methods and target antigen for production of vaccines. The antigenic components of intact and trypsin-treated T.evansi were identified. Seventy protein bands were detected in the trypanosomal preparations using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Twenty nine of these components were identified as antigens using immunoblotting against sera from infected rabbits and rabbits immunised with a range of soluble parasite materials. The solubilisation procedure and immunisation method affected the number of antigens recognised by these sera. The trypsin-treated parasites failed to produce any antibody response as measured by immunoblotting and ELISA. Two antigens identified by immunoblotting were selected for further study, a surface associated component of ˜ 52 k.Da which was recognised by the early infection serum and a non-surface component of ˜ 42 k.Da which was recognised strongly by sera from infected rabbits and rabbits immunised with the parasite soluble materials. A third antigen of molecular weight of ˜ 72 k.Da recognised by monoclonal antibodies raised to the intact living trypanosomes was also included in the study.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available