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Title: An in vivo and in vitro investigation of development of the cerebral neocortex in the mouse brain
Author: Edgar, Julia M.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1999
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I used 5-bromo-2-deoxyuridine (BrdU) to label proliferating neuronal and glial precursors in vivo and used immunohistochemical techniques to investigate their distribution within the developing mouse neocortex at embryonic and early postnatal stages. I developed a double labelling protocol to study the antigenic characteristics to the glial cells. I showed how neuronal and glial precursors invade the developing neocortex, characterised the glial cells in terms of their antigenic properties and investigated their proliferative behaviour. To investigate the factors that regulate the formation of the cortical layers, I developed a tissue culture technique and used BrdU labelling to study a population of neuronal precursors that give rise to cortical layers III and IV. These cortical layers receive direct thalamic innervation in vivo. I characterised the culture system and showed that it provided a satisfactory model for the study of the cell migration. I then showed that the thalamus was important for the normal migration of layer III/IV cells. I also showed that although new cortical layers did not form in vitro, the ability of cells to maintain a relatively normal lamination could be improved by the addition of tetrodotoxin (TTX) to the culture medium. I also showed that TTX treatment helped preserve the normal distribution of Cajal-Retzius cells in the marginal zone. To ascertain if there was an in vivo correlate for my in vitro results, I used an immunohistochemical technique to characterise the thalamus, thalamocortical innervation and Cajal-Retzius cells in the mutant mouse Small eye (Sey), in which migration of late born cortical cells is aberrant. The results of both the in vitro and in vivo investigations suggest a role for the thalamus in the normal formation of cortical layers III and IV.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available