Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.649711
Title: Factors affecting telomere structure and function in the fission yeast Schizosaccharomyces pombe
Author: Drakeford, Clare E.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2002
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Abstract:
Telomeres are complex nucleoprotein structure formed at the ends of linear eukaryotic chromosomes, which consist of a repetitive short nucleotide repeat and associated proteins. Telomeres are important for the stability of the genome, complete replication of the ends of chromosomes. In addition the proteins associated with telomeres form a protective cap, which camouflages the ends of the chromosome so that they are not recognised as double-strand breaks. This cap also protects chromosome ends from nucleolytic degradation and blocks the formation of end-to-end fusions. Failure to maintain telomeres may result in genetic instability and uncontrolled cell division and is implicated as a major factor in tumourogenesis. Chromatin at telomeres is assembled into a transcriptionally repressed structure, which leads to the reversible silencing of genes placed in their vicinity. The functions of telomers are conserved across many species. Using a variety of genetically tractable organisms such as Schizosaccharomyces pombe and Saccharomyces cerevisiae to dissect telomere function allows scientists to compare and contrast the formation of telomers in eukaryotes. In this thesis I set out to identify novel factors involved in telomere structure and function in the fission yeast S. pombe. Two genetic screens were performed using alleviation of repression of telomeric marker genes to isolate mutants of interest. In the first screen, spontaneous mutagenesis and UV induced mutagenesis were used to create an extensive collection of mutants. In an attempt to overcome problems in identifying affected genes by complementation a second screen was performed using insertional mutagenesis. A number of the mutants resulting from these screens were assigned to complementation groups by genetic crossing and their phenotypes characterised using a variety of techniques including Southern blotting, spore analysis, Immunolocalisation and live analysis. Expression of normally silent telomeric genes was analysed by growth assays and quantification of transcript levels. The screening techniques and analysis of mutants are discussed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.649711  DOI: Not available
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