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Title: A study of ovine β-lactoglobulin transgene expression in the mouse mammary gland
Author: Dobie, Kenneth W.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1996
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Lines of mice carrying an ovine β-lactoglobulin (BLG) transgene secrete large quantities of BLG protein into their milk. To explore the stability of transgene expression, a systematic study of expression levels in three BLG transgenic mouse lines was performed. Unexpectedly, two of these lines exhibited variable levels of transgene expression. Copy number within lines appeared to be stable and there was no evidence of transgene rearrangement. Studies on the most variable line showed that BLG production levels were stable within individual mice in two successive lactations. Backcross experiments demonstrated that the genetic background did not contribute significantly to the variation of expression levels. Tissue in situ hybridisation experiments revealed mosaic patterns of transgene expression within individual mammary glands from the two variable lines; in low expressing animals, discrete patches of cells expressing the transgene were observed. The concentration of transgene protein in milk reflected the proportion of mammary epithelial cells expressing BLG mRNA. Furthermore, in situ hybridisation to metaphase chromosomes indicated that the transgene arrays in both these lines are situated close to the centromere. These experiments suggest that variable mosaicism of transgene expression among individuals within a transgenic line is a consequence of the chromosomal location and/or the nature of the primary transgenic integration event.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available