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Title: Cell division in Escherichia coli : the expression and regulation of division genes
Author: Dewar, Susan J.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1988
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The four essential cell division genes ftsQ, ftsA, ftsZ, and envA are arranged sequentially within a large cluster of genes required for cell envelope growth and form. The bacteriophage λJFL100 carries the 1.8 kilobase EcoR1-HindIII chromosomal restriction fragment which spans the ftsQ and part of ftsA coding sequences. This fragment contains at least two promoters; one within ftsQ is required for the expression of ftsA and one within ftsA is required for expression of ftsZ. The fts fragment is cloned upstream of a lacZ gene so that transcription originating from within the fragment leads to the production of β-galactosidase. Expression from the fts promoters in cells lysogenic for the phage is shown to be derepressed in the absence of FtsA protein. The results presented here suggest that transcription from these promoters is linked to the cell's periodic requirement for FtsA protein during septum formation. The exact positions of the promoters within the group are not yet known although their approximate locations have been determined by promoter assay and sequence analysis. An attempt has been made to define the position of the ftsA promoter more precisely by in-vitro transcription from short, defined templates and by the sequential deletion of ftsQ. Serial deletion through ftsQ has revealed what appears to be a complex upstream regulatory region, reminiscent of a eukaryotic enhancer element, which influences the expression of ftsA. A model is presented for the transcriptional control of ftsA expression.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available