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Title: Molecular approaches to the improvement of viral safety of blood and blood products
Author: Davidson, Fiona Jane
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1995
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This thesis is concerned with the detection of viruses in blood and blood products, with the general aim of improving the safety of blood donations and factor concentrates for clinical use. The prevention of virus transmission by blood and blood products is currently based on the screening of blood donations for virus specific antibody or antigen and by incorporation of virus inactivation procedures in the manufacturing process. In this study virus detection was by reverse transcription (RT) of virus RNA (where appropriate) followed by amplification of cDNA of virus DNA by the polymerase chain reaction (PCR). The viruses studied were hepatitis C virus (HCV), parvovirus B19 and hepatitis A virus (HAV). For HCV, the relationship between detection of anti-HCV by recombinant immunoblot assay (RIBA-2) and viraemia was examined and a good correlation (84.4%) between a positive result in the RIBA-2 and detection of HCV by PCR was observed. However 5.4% of donations that were RIBA-2 indeterminate were PCR positive, demonstrating that PCR can be useful in the confirmation of ambiguous serological results. An assay system was developed that identifies different HCV genotypes by restriction fragment length polymorphisms (RFLP) in the 5' non-coding region of the genome. In a worldwide study of the distribution of HCV genotypes in blood donors, two new genotypes were discovered. Distinct geographical distributions of HCV genotypes were observed; HCV types 1, 2 and 3 were widely distributed while genotypes 4, 5 and 6 were detected exclusively in donors from one country only.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available