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Title: Characterisation of the Musca domestica (Diptera) homologue of the Drosophila melanogaster segment polarity gene patched, and the cloning of a cDNA fragment, PD, from Acheta domesticus
Author: Daniels, Graeme P.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1998
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The Drosophila segment polarity gene, patched (ptc), encodes a transmembrane protein that is the receptor for the hedgehog intercellular signalling molecule. Mutations in the human ptc gene, have been shown to be responsible for Gorlin's syndrome and a predisposition to nevoid basal cell carcinomas. In this study, partial clones of patched homologues were isolated from the long germ band developing house fly, Musca domestica (Diptera), and the intermediate germ band developing house cricket, Acheta domesticus (Orthoptera). Sequence data obtained from the Musca domestica clones showed a high degree of similarity with the Drosophila ptc gene sequence, indicating that the Musca domestica clones contain real ptc homologues. The Acheta ptc fragment was cloned using degenerate PCR, and sequence data has shown it has a high degree of similarity to the comparable regions of other ptc homologues. Two phagemid clones were also isolated from an Acheta cDNA library using a strategy designed to isolate Acheta ptc. One of these clones, PD, was used to create RNA in situ hybridization probes, and its expression was examined during Acheta embryogenesis, although it was later shown that PD was not an Acheta ptc homologue. Expression of the Musca ptc homologue was examined during early development using RNA in situ hybridisation, and immunohistochemistry. These studies have shown that the expression of ptc during Musca domestica development is very similar to ptc expression patterns seen during the early development of Drosophila, suggesting that ptc may be fulfilling a similar role in both species.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available