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Title: Mechanisms of injury in experimental glomerulonephritis
Author: Sharp, Phoebe
Awarding Body: Imperial College London
Current Institution: Imperial College London
Date of Award: 2013
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Proliferative glomerulonephritis (GN), seen in patients with Goodpasture's disease or systemic lupus erythematosus (SLE), is characterised by glomerular infiltration of macrophages and T cells as well as proliferation of intrinsic renal cells. Significant insights into the pathogenesis of GN have been obtained through the use of experimental rodent models, such as nephrotoxic nephritis (NTN). In this thesis I have investigated the role of two important immunological molecules in NTN: Fas ligand (FasL) and Fc gamma receptor IIB (FcγRIIB). FasL is a well-known inducer of apoptosis in cells expressing its receptor Fas; however FasL also mediates non-apoptotic pro-inflammatory responses. FasL is widely expressed throughout the hematopoietic system but is also expressed within healthy and diseased kidney. I describe a novel role for FasL in the promotion of NTN independent from antibody and T cell responses. This pathological effect correlates with enhanced glomerular inflammation and appears to be dependent on FasL expression on both circulating leukocytes and intrinsic renal cells. Additionally, I have shown FasL-defective mesangial cells have impaired signalling through the IL-1R with a reduction in MCP-1 production. FcγRIIB is the sole inhibitory Fc receptor for IgG and is involved in the negative regulation of B cells and cellular activation. FcγRIIB is widely expressed throughout the hematopoietic system but is also expressed on mesangial cells within the kidney. FcγRIIB deficiency greatly exacerbates NTN. Here I use cell-specific deletion to demonstrate the critical importance of FcγRIIB expressed on myeloid cells rather than B cells in the protection from NTN. Further to this, I highlight a role for FcγRIIB on intrinsic renal cells, possibly mesangial cells, in the protection from NTN. Overall, these data widen our knowledge on the pathogenesis of GN and open up possibilities for finding future novel treatments.
Supervisor: Tarzi, Ruth; Cook, Terence; Pusey, Charles Sponsor: Arthritis Research UK
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available