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Title: Characterisation of potentially host-protective material from the abomasal parasite, Teladorsagia circumcincta
Author: Craig, Hannah L.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2004
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The main aims of this study were to identify and characterise proteins from T. circumcincta that may induce a protective immune response in the host and to learn more about the biology of the worm. In order to identify possible protective antigens, a complementary DNA (cDNA) library prepared from adult worms was screened with serum from an animal that was protected against a single challenge infection after vaccination with a T. circumcincta protein fraction (S3 TSBP). Forty five immunopositive cDNA clones were identified, of which sixteen had homology to galectin. Of the remaining clones, the majority shared homology with two metabolic enzymes, methylmalonate semialdehyde dehydrogenase and 10-formyltetrahydrofolate dehydrogenase, that have not been characterised in nematodes. A single clone with homology to the antioxidant enzyme, catalase, was also identified. These three enzymes were selected for further investigation on the basis of their roles in nematode metabolism and therefore, their potential as vaccine candidates. Characterisation of T. circumcincta excretory/secretory material (ES) was also performed. L4 and adult worms were cultured in vitro and the proteins released were separated by 1D electrophoresis and analysed by Tandem Liquid Chromatography Mass Spectrometry and N-terminal sequencing. This identified proteins showing similarity to, amongst others, metabolic enzymes, structural components, antioxidants, globin-like proteins and cysteine proteases, present in online databases but not previously characterised in T. circumcincta. This study has identified several novel T. circumcincta proteins that may have potential as future vaccine or drug targets. It has also provided further information regarding the biology of the worm.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available