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Title: Virus-induced apoptosis of glial cells
Author: Cotterill, C.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2005
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The aim of this thesis is to determine the fate of oligodendrocytes upon SFV infection, to ascertain the nature of any cell death and, if observed, to determine whether this is dependent on cell maturation state. The outcome of SFV infection of oligodendroglia derived from mixed primary cultures was dependent on the degree of cellular homogeneity. Immunocytochemical studies revealed that immature O-2A progenitors died rapidly, as revealed by double staining for caspase-3 and early O-2A cell markers. The susceptibility of mature oligodendrocytes was found to be dependent on cellular environment. Mature oligodendrocytes were equally susceptible to virus-induced apoptosis as immature progenitors when cultured in isolation i.e. as enriched purified populations. On the contrary, cultures of mature oligodendrocytes maintained in the presence of other glial cells, or in contact with extracellular matrix molecules present in vivo, survived infection for the period under study; two weeks. The fate of the mature oligodendrocyte was further investigated in the corpus callosum in vivo using sections from SFV-infected adult Balb/c and nu/nu mice. Dual immunolabelling for virus and apoptosis (TUNEL staining) revealed an active period of cell death between PID 4 and -6. Double immunofluorescence for 2’,-3’-cyclic nucleotide 3’-phosphodiesterase (CNPase) and SFV or caspase-3 visualised by confocal microscopy indicated that although mature oligodendrocytes were infected with SFV they did not die by apoptosis. In conclusion, this study presents evidence that isolated enriched cultures of mature oligodendrocytes are susceptible to apoptosis as are their immature counterparts. Paradoxically, mature oligodendrocytes studied in vivo or maintained in the presence of other glial cells factors in vitro, did not undergo virus-induced apoptosis, unlike their immature relatives.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available