Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.643308
Title: Are CYCLOIDEA-like genes involved in the control of floral zygomorphy in Schizanthus wisetonensis?
Author: Coenen, Karine
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2004
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Abstract:
In Antirrhinum majus (Scrophulariaceae), floral dorso-ventral asymmetry (monosymmetry) is controlled by two genes belonging to the TCP gene family, CYCLOIDEA and DICHOTOMA. My project was to investigate if putative orthologs of CYC and DICH have a conserved role in Schizanthus wisotonensis (Solanaceae), a species also bearing monosymmetrical flowers. To do so, TCP genes from S. wisotonensis were isolated and their expression pattern was characterised in both a wild type and a mutant plant with decreased dorso-ventral asymmetry. In the first chapter of results, a thorough morphological description of floral development in S. wisotonensis was carried out using the support of Scanning Electron Microscopy and quantitative analysis. The development of wild type S. wisotonensis was then compared to that of the mutant. In the second chapter of results, the cloning of 6 TCP genes in S. wisotonensis is reported (SCHCYCI-6). To identify the putative orthologs of CYC, a phylogenetic analysis was carried out using sequence information from other angiosperm TCP genes. This study showed that SCHCYCl, 2 and 3 are the most likely candidates to control dorso-ventral asymmetry in S. wisotonensis. Finally, in the third chapter of results, the study of expression patterns obtained for SCHCYCI-3 using RNA in situ hybridisation in both the wild type and the mutant is reported. The results obtained from the morphological description and the study of gene expression patterns suggest that (i) the establishment of dorso-ventral asymmetry in S. wisotonensis follows a different dynamic to that in A. majus (ii) SCHCYCI may have a function related to that of CYCLOIDEA and DICHOTOMA but unlike for A. majus, it is likely to be restricted to the androecium (iii) CYC-like genes (i.e. SCHCYC2-3) may have a function in the development of the inflorescence where they are expressed in overlapping domains.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.643308  DOI: Not available
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