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Title: A study of experimental heterotopic ossification induced by urinary bladder epithelium
Author: Callis, Paul David
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1979
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Heterotopic ossification can be induced experimentally following the implantation of certain types of epithelium, or devitalized hard tissues. In this thesis auto-transplants of guinea pig urinary bladder epithelium to the anterior abdominal wall were examined. The animals were killed at intervals of up to six months, and the transplant area studied with the light and electron microscopes. All histological material was serially sectioned and measured with a semi-automatic image analysis system to assess accurately the development of cyst and bone. Maps of the cyst wall were drawn from section tracings and all results were analysed statistically. Fibrin clots formed between transplant mucosa and site musculature shortly after operation. At Day 2 the epithelium of the transplant edge began to proliferate and extended between the clot and transplant mucosa to form a cyst by Day 8. Meanwhile the clot was replaced by fibrous tissue, within which at Day 10 the first osteogenic foci were found. During the next seven days, matrix vesicles could be observed at the surface of developing bone. From Day 17 onwards osteoclasts were present. Occasionally after Day 17, beneath the original transplanted epithelium, small osteogenic areas were observed. The cyst contained small tetrahedral crystals in some cases, between Day 10 and Day 100. Within the epithelium of the cyst lining maturation changes were observed from Day 9 and a typical urinary bladder epithelium had developed by Day 40. Several forms of epithelial aberrancy were found; outgrowths beneath the cyst lining (some associated with bone formation), and three forms of hyperplasia. It appeared that at about Day 40, areas of epithelium overlying the bone were shed and yet only beneath such areas did bone formation occur.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available