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Title: Design, construction, modelling and control of a dual-hollow fibre bioreactor for hybridoma cells
Author: Burns, John W.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1991
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This thesis describes research carried out by the author between 1988 and 1991 at the Department of Chemical Engineering, University of Edinburgh, under the supervision of Dr Donald Glass and Dr Bruce Ward. The aim of the research was to design, build, operate and control a dual-hollow fibre bioreactor. The principle behind the design is that of the blood supply system in animals. The nutrients are supplied in one set of fibres to the growth region, similar to the arteries in the blood system, and another separate set of fibres takes waste products away from the growth region, in a manner analogous to the venous system. The design, construction and operation of the bioreactor is described. The development of novel building techniques are explained, covering new ground in fibre bioreactor construction. The monitoring equipment required is described with a number of successful experimental runs demonstrating the data collection capabilities of the apparatus. During the research, areas of work not initially envisaged were explored, with the aim to provide a basis for future control strategies. This included the development of a fibre testing rig, so that different fibres and various medium preparations could be tested outside a reactor system. This was done due to the lack of basic information available on fibre performance. This leads into work on the modelling the bioreactor by means of a numerical solution run on a computer. The model provides new areas of simulation, the fouling of fibres and the changing nutrient concentrations supplied to the bioreactor. The work is now at a stage where experimental work and modelling work should be brought closer together to help understand problems experienced in both areas.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available