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Title: Using 3D reconstruction to analyse early mouse development
Author: Brune, Renske M.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2002
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Our current description of mouse developmental anatomy has mainly been based on studies using SEM, confocal, light or dissecting microscopy and, as the resulting picture gives an incomplete understanding of 3D morphological relationships within the embryo, it is inadequate for current molecular and genetic studies. The work reported here sets out to improve the situation by describing novel aspects of mouse developmental anatomy obtained from the analysis of 3D digital reconstructions of serial sections of mouse embryos at embryonic days 1-9 (E1-9) within which all anatomically defined tissues were delineated. These embryos were carefully selected for being representative of their developmental stage and reconstructions were checked for normal representation of tissues. The reconstructions contain histological detail accessible at any angle through the reconstruction within the reconstructions, while surface rendering software can display 3D tissue organisation. These reconstructions have been used to explain three particular aspects of mouse development: geometric relationships in the early stages of mouse development, and morphological events that accompany turning, and the relative growth rates of the key tissues during early embryogenesis and organogenesis. The geometric relationships of the reconstructions of E1-9 were analysed and compared to the existing description of mouse occurs earlier than hitherto reported, namely at E5-6.5. Two blastocysts are bilaterally symmetric but only the earlier stage shows a tilt in the inner cell mass with respect to the embryonic-abembryonic axis. The tilt in the ectoplacental cone with respect to the latter axis is not as a consistent angle, but does coincide with an asymmetry in the extraembryonic ectoderm that could be explained by the tilt of the cone. During early organogenesis, the myocardial walls, cardiac jelly and endothelial lining are congruent. The vascular system develops in isolated sacs that are inline and link up later. There is no obvious morphological asymmetry between left and right components of structures.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available