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Title: A study of the organisation of the chicken genome
Author: Bruley, Charlotte Kate
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1999
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Avian genome mapping efforts have concentrated on the domestic chicken (Gallus gallus) due to its economic importance and as a model of vertebrate development. Its karyotype consists of a 6 large macrochromosomes and 33 small microchromosomes and its genome is 1.2 x 109 bp in size. Previous work showed microchromosomes are GC rich and CpG island rich and macrochromosomes are AT rich and CpG island poor. This suggests that the microchromosomes, though small, are gene dense and the macrochromosomes are less gene dense. The question of are microchromosomes gene dense and macrochromosomes, by comparison, less gene dense was asked. Microchromosomes were found to be approximately twice as gene dense as macrochromosomes. The distribution of the avian retrotransposon repeat family Chicken Repeat 1 (CR1) was used to test the hypothesis of if microchromosomes are gene dense, they should have fewer mobile repeats because they contain less 'junk' DNA. A two-fold difference in CR1 repeat density macrochromosomes Vs microchromosomes was observed. This, combined with the numbers of CR1s calculated, supported the hypothesis. In addition to this, new CR1 subfamilies were assigned. Conservation of the linkage group 5' TH-INS-IGF-II-3' in chickens was studied. Work was carried out to determine the order of these genes. The conserved linkage of the genes TH and INS was established but their order could not be determined.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available