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Title: The production and study of Theileria annulata macroschizont infected cells : relating to MHC class II expression, T cell stimulatory ability, cytokine mRNA production and their use as vaccines
Author: Brown, David John
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1997
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Theileria annulata is a protozan parasite of cattle of the genus Apicomplexa, which causes the life threatening disease Tropical Theileriosis. In susceptible animals progression of this disease can be rapid with death occurring within 14 to 20 days post infection. T.annulata infection is characterised by the proliferation of macrophages (Mθs) infected with the macroschizont stage of the parasite's life cycle. This stage is closely associated with pathology but may also be the main stage against which animals mount a protective response. The aims of this thesis are to (1) investigate the causes and mechanism of pathology, (2) study ways in which the pathology of T.annulata infection can be decreased and the protective response boosted, (3) investigate and improve methods of vaccine production and selection. In vitro, T. annulata infected cells possess augmented antigen presenting function and also the ability to activate resting naive/memory autologous T cells in a contact dependent manner. In vivo infected cells have been shown to congregate initially in the medulla of the draining lymphnode, where they associate with and activate T cells. This thesis investigates T cell stimulatory ability of T. annulata infected cells. Clonal populations of T. annulata infected cells were generated from CD14+ monocytes and Mθs. cultured and used to stimulate autologous native T cells. T cell proliferation assays showed the clonal cultures possessed different T cell stimulatory abilities. Previous work showed that infected cells expressed elevated levels of MHC class II molecules. These molecules are involved in the stimulation of T cells and the possibility that the signals involved in nonspecific T cell activation were linked to MHC class II expression was investigated. Expression levels of MHC class II molecules by the clonal cultures showed data collected did not correlate with the T cell stimulatory ability of the infected cells. After this finding the production of cytokine mRNA by infected cells was investigated. Cytokines are known to play major roles in the control of cellular activation/proliferation and immune responses.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available