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Title: Studies on carotenoid production in cultures of Bixa orellana
Author: Boyd, Margaret Gray
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1991
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The aim of this project was to study the production of carotenoid pigments in cultured cells of Bixa orellana to develop cultures with a high yield of product. Orange/red pigmented cells were detected in phloem and cortical tissues of stems, petioles and roots of B.orellana and in the phloem and spongy mesophyll of leaves. Bixin, β-carotene, lutein and several unidentified pigments were present in the vegetative tissues whereas only bixin was present in the seeds. Both callus and suspension cultures of B.orellana were established. The pigment levels in the callus cultures were very low and appeared to decline as the cultures aged. Attempts to increase pigment production in callus using standard procedures such as reduction of auxin, removal of nitrogen or phosphate, addition of picloram and cell selection methods were not successful. However, pigmented cells were detected in newly initiated callus from root explants and the number of these cells increased as the callus was established. Manipulating the culture medium using 8% instead of 3% sucrose or by removing nitrogen and/or phosphate decreased the growth of newly initiated callus tissue and increased the pigment on a per mg. fresh weight basis compared to the control. Carotenoid production in B.orellana suspension cultures was shown to occur in the lag and early growth phases of batch growth. Depletion of nitrogen or an increase in the sucrose concentration from 3% to 8% resulted in an increase in pigment accumulated in suspension cultures. An increase in the sucrose concentration in the culture medium delayed cell division and growth of cultures and, within limits, the higher the sucrose concentration the longer the delay before an increase in cell number could be detected.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available