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Title: A study of the Drosophila 'heat-shock' genes
Author: Bower, Daphne
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1979
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It has been observed that during exposure to unusually high temperatures, and also under other conditions of stress, the normal mRNA and protein synthesis of Drosophila cells is replaced by expression of a sma11 number of genes; this has led to the speculation that these genes are under some form of coordinate control. In this study, the differential expression of these genes during development of D. melanogaster has been demonstrated, using as a probe a mixture of "heat shock" mHNA complementary DNA sequences. It was found that a variable proportion of the "heat shock" genes are measurably active throughout development, when assayed by hybridisation techniques. Since the probe was partially fractionated the detected differences in gene expression at different stages were amplified. Complementary DNA sequences were then cloned to yield pure probes for individual heat shock sequences, which could be used in future experiments to precisely follow the expression of single genes; in addition these cloned sequences could be used to identify complementary genomic DNA sequences.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available