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Title: Nucleotide sequence of a transcription termination region in coliphage T7
Author: Boothroyd, John C.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1979
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A detailed physical map of restriction endonuclease cutting sites has been determined for the region surrounding the transcription-termination site at the end of the early operon of coliphage T7. Using this information and the chain-terminating method for sequencing DNA, a nucleotide sequence 370 base-pairs in length has been determined corresponding to the DNA between 18.88 and 19.81 on the T7 physical map. By comparison with the data of others, including nucleotide sequences for regions of known physiological importance, several interesting functional sites have been tentatively identified within the sequence presented. These include: 1) the termination site (map position 18.96) for transcription of the early operon by E.coli RNA polymerase; 2) two sites (19.35 and 19.65) likely to act as promoters for the initiation of class II transcripts by T7 RNA polymerase; 3) two regions (18.89-19.31 and 19.34-19.61) each of which includes a plausible ribosome-binding site and which probably code for a pair of previously unreported class II genes tentatively designated genes 1.4 and 1.5, respectively; 4) the ribosome-binding site (position 19.65) and initial 18 codons of the class II gene 1_7. A particularly striking feature of this sequence is the frugal arrangement of several of the proposed functional sites so that two perfect, direct repeats of 21 base-pairs contain the stop-codons for the putative genes 1.4 and 1.5, the two class II promoters, and the ribosome-binding sites for genes 1.5 and 1_7i respectively.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available