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Title: Population diversity in Theileria annulata in Tunisia
Author: Ben Miled, L.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1994
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Tropical theileriosis, caused by the haemoprotozoan parasite Theileria annulata, is one of the major threats to cattle health in Tunisia. The aim of the study described in this thesis was to assess the extent of diversity in T. annulata parasite population from within a single country in order to provide a better understanding of the epidemiology of the disease and biology of the parasite. The work also provided useful background information to vaccine development studies in Tunisia. Different T. annulata stocks, isolated from different bioclimatic zones in Tunisia were characterised using monoclonal antibodies (MAbs), isoenzyme and DNA analyses and compared with each other and with a number of recognised laboratory stocks from other theileriosis endemic areas of the world. The study comprises seven chapters. In the first, an introduction to the literature describing the parasite T. annulata and the disease it causes are presented and the epidemiology of tropical theileriosis is discussed with particular regard to the situation in Tunisia. Finally an overview on diversity in protozoan parasites, including Theileria, is given to emphasise the rationale behind the present study. The next chapter details how the biological material, including 51 field isolates, was generated for the three parasite life-cycle stages, sporozoites, piroplasms and schizonts. In the third chapter the production of antischizont MAbs and their use as immunological makers to reveal differences between stocks of T. annulata is described. The fourth chapter demonstrates the use of a biochemical marker, glucose phosphate isomerase isoenzyme to study diversity in T. annulata isolated in Tunisia.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available