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Title: The fate of the inflammatory macrophage with resolution of inflammation
Author: Bellingan, Geoffrey John
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1996
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Neutrophils have been shown to undergo apoptosis and be ingested by macrophages (Mo) thus providing a unique method for their clearance that does not induce further pro-inflammatory signals. This work investigated the fate of the Mo during the resolution of inflammation. To study the fate of inflammatory Mo during the resolution of inflammation in vivo, a murine model of resolving peritonitis was developed in which the fate of semi-allogeneic adoptively transferred donor inflammatory Mo could be tracked. Red fluorescent labelled donor H-2dk inflammatory Mo were transferred into the peritoneal cavity of recipient H-2kmice which were at the same stage of resolving inflammation. Donor, recipient, and donor cells which had been phagocytosed by recipient Mo, could be distinguished by two colour flow cytometry using a FITC conjugated anti H2d monoclonal antibody, the phagocytosed cells being red fluorescent labelled but H-2d negative. Adoptively transferred live inflammatory Mo disappeared steadily from the peritoneal cavity, being undetectable 96 hours post transfer and minimal phagocytosis was evident during this process. In contrast, fixed donor Mo were rapidly phagocytosed by the recipient Mos and these cells could be detected up to a week later. These data suggested that adoptively transferred live inflammatory Mo emigrated from the peritoneal cavity during the resolution phase of inflammation. Labelled cells were detected specifically in the draining lymph nodes and later in small quantities in the liver and spleen but not in a range of other tissues. Thus by contrast with the neutrophil, which meets its fate during the resolution of inflammation locally by undergoing apoptosis and being ingested by Mos, the inflammatory Mo appears to emigrate from the inflamed site to the local lymph nodes where it then meets its fate by as yet obscure mechanisms.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available