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Title: Homeobox containing genes in the leech
Author: Baxter, Euan W.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1993
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Leech embryos have been used in developmental studies for over 100 years and continue to be used because of a number of unique and useful features. There is a large amount of detailed information on the highly stereotyped cellular events that take place during leech development, and recent work has shown that it is possible to study some of the genes in leech that have been shown to exert developmental effects in other species. This thesis presents that sequence of two fragments of homeobox genes which were generated from leech Helobdella robusta by polymerase chain reaction. These genes have been named Lox 7 and Lox 8. Some preliminary in situ hybridization data suggests that the Lox 7 gene is expressed in the germinal plate of stage 9 embryos. A genomic library was constructed from Helobdella robusta DNA and screened with the Lox 7 and Lox 8 fragments. A control screening was carried out with Lox 2. These experiments proved that the Lox 7 and Lox 8 genes were not represented in the library. Positives were obtained from the control screening of the Helobdella robusta genomic library and were investigated further. Evidence is presented that some of these clones do not contain the gene used to make the probe (Lox2), but may contain other homeobox genes. Positive clones were gained from the screening of a Helobdella robusta cDNA library with the leech engrailed gene. The clones show that the mRNA from the engrailed gene is alternatively spliced.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available