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Title: Seed viability and in vitro culture of Afrocarpus gracilior and germination studies on Ekebergia capensis and Pygeum africanum
Author: Balcha, Girma
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1996
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The conditions which prolong viability and the low germination rate of seeds of Afrocarpus gracilior were studied. Different seed lots were stored under various moisture content and temperature combinations. The two seed lots tested had different initial viabilities, probably due to different pre-storage treatments, but generally similar storage requirements to maintain viability at a reasonable level. Tetrazolium tests gave consistently higher viability estimates than germination tests but were well correlated with the latter. Intact seeds were found to have a very low germination rate. However, removal of the seed coat and germination at 30oC resulted in germination rates of up to 80% after 30 days. The seed coat was found to contain a very powerful inhibitor of germination of Triticum seed. Studies were made on the germination of seeds of Ekebergia capensis and Pygeum africanum. Intact seeds of E. capensis germinated at about 52% under optimum conditions and slight damaging of the seed coat halved the germination rate. Intact seeds of P. africanum germinated at about 20% under optimum conditions and slight damaging of the seed coat doubled the germination rate. Shoots of A. gracilior were found to grow well in vitro on hormone free medium but did not produce axillary buds during normal growth and development. However, it was found that decapitation resulted in the formation of axillary buds at or near the top of the shoots which subsequently developed into shoots. Treatment of intact or decapitated shoots with benzyladenine resulted in the initiation of numerous buds but it proved impossible to extend them into shoots. Extending shoots rooted spontaneously on hormone free medium and were also rooted ex vitro in compost under non-sterile conditions and the rooted plants were weaned to greenhouse conditions.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available