Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.641189
Title: Factors associated with uterine functions in the mare
Author: Bae, S. E.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2002
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Abstract:
Delayed uterine clearance is the most common cause of subfertility in the mare. Previous studies have shown that mares that accumulate intrauterine fluid have impaired myometrial activity. Uterine contractility is a complex mechanism controlled by coordination of myogenic, neurogenic and hormonal factors. The aims of this study were to investigate the factors which are associated with uterine contractility in mares. The uterus of the mare was well supplied by a variety of nerve fibres. Using general neuronal marker, PAN-N and PGP 9.5, it showed a general view of equine uterine innervation. Within the regions of the uterus, nerve density score for both PGP-immunoreactive (IR) and PAN-N-IR was greatest in the myometrium. There were no marked differences between the supply to uterine horn or body, but nerve density was significantly greater (P<0.05) in the cervix. The nerve supply was predominantly adrenergic and was distributed throughout all regions of the uterus. With adrenergic nerves, the density score was the greatest in the myometrium within the structure and in the cervix within the regions. Peptidergic nerves were also seen and were slightly denser in the cervix than in the uterine horn or uterine body. Among the peptidergic nerves, neuropeptide Y was the most abundant, whereas vasoactive intestinal polypeptide and calcitonin gene-related peptide were less frequent. Substance P was rarely observed. The presence of oxytocin and neurophysin in the uterus was demonstrated using immunohistochemistry. Ultrastructural studies showed that these hormones were stored in the secretory vesicles of the luminal secretory cells and the secretory cells in the superficial endometrial glands. Ciliated cells in the luminal epithelium and endometrial glands did not show any positive staining for either oxytocin or neurophysin. Mean plasma oxytocin concentrations in genitally-normal mares (n=5) were significantly higher (P<0.02) in oestrus (day -5 to day -2) than the day of ovulation (day 0). On the day 1 post ovulation, mean plasma oxytocin concentrations were the lowest level. Plasma oxytocin concentrations in day 2 oestrus were significantly higher (P<0.01) in genitally-normal mares (n=5) than in mares with delayed uterine clearance (n=5).
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.641189  DOI: Not available
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