Use this URL to cite or link to this record in EThOS: http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.641083
Title: Intestinal antibodies and intra-epithelial lymphocytes in potential coeliac disease
Author: Arranz, Eduardo
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1994
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Abstract:
This work refers to the immunological abnormalities found in coeliac disease and other patients with similar gastro-intestinal symptoms but a morphologically normal biopsy. I assessed the use of jejunal fluid for studies of intestinal immunity, possible leakage of plasma proteins, and proportion of locally produced sIgA. Previous work with Dr. O'Mahony showed that DH patients without enteropathy, had an abnormal pattern of intestinal antibodies, as found in untreated and treated coeliacs after mucosal healing. This led to the characterization of the CIA pattern, a potential marker of latent coeliac disease. I examined its frequency in a large cohort of patients referred for diagnostic jejunal biopsy. Jejunal fluid immunoglobulins and IgA and IgM antibodies to GLI, OVA, and BLG; and serum IgG and IgA antibodies to GLI, were measured by ELISA. Between 15-20% of the patients were CIA positive, some of them with high IEL counts in jejunum. Studies of epithelial TCR τδ cells in frozen sections showed a correlation between high IEL τδ counts and CIA pattern. Almost 40% of patients have one or more immunological indices of potential coeliac disease, but no other associations were found. I studied the immunopathogenesis of the CIA pattern, by counting plasma cells, measuring cytokine levels in secretions, and cell activation markers in mucosa. Trials of gluten-free diet have shown clinical gluten-sensitivity without enteropathy in some patients. One of them, who was positive for three markers, has developed subtotal villus atrophy after taking extra gluten.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.641083  DOI: Not available
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