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Title: Regulation of mitochondrial dynamics in adipose tissue
Author: McCarthy, Ciara M.
ISNI:       0000 0004 5349 9845
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 2014
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This thesis advances the understanding of mitochondrial dynamics in adipocytes. Chapter 1 outlines mitochondrial dynamics, health, function and quality control in the context of obesity and T2DM. Chapters 2 and 3 are concerned with characterising mitochondrial dynamics in human abdominal subcutaneous adipose tissue and how the balance of this process may be augmented by adiposity and bariatric surgery. As an extension of this, Chapter 4 explores the potential regulatory role of p38 within adipogenesis in the 3T3-L1 cell line model together with the interplay between p38 and mitochondrial dynamics throughout differentiation. As part of this study, the impact of modulating p38 by chemical means or via the introduction of an overexpression vector was used to investigate the functional consequences of this gene on mitochondrial bioenergetics. The subtle balance of mitochondrial fusion and fusion markers is critical, not only for controlling the abundance and bioenergetic health of mitochondria but also, for overall cellular health. Finally, Chapter 5 extends analysis of the regulatory role of p38 on mitochondrial dynamics through computational modelling of the p38β. From the entire AstraZeneca compound collection, a series of novel and putative p38β inhibitors were selected for further functional assays to assess the effect of these compounds on mitochondrial bioenergetics in 3T3-L1.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QP Physiology