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Title: The interaction between Bacteroides (cell surface antigens) and the immune system in health and disease
Author: Allan, Elizabeth
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1995
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The aim of this thesis was to examine the interaction between Bacteroides species, in particular their cell surface antigens, and selected aspects of the immune system, both in health and disease. Complement-mediated killing of Bacteroides species was examined. It was found that growth environment could have a profound effect on serum sensitivity of certain strains. When grown in proteose peptone-yeast extract medium, all 12 strains tested were sensitive to complement. However, when grown in Van Tassell and Wilkins' medium, six of the strains became markedly more complement-resistant, and when grown in heat-inactivated sheep serum, five of these six strains became totally resistant. Immunoglobulin G (IgG) levels to bacteroides LPS, and to a cocktail of rough LPS from three enterobacteria and P. aeruginosa were examined in enzyme-linked immunosorbent assay (ELISA), firstly in healthy individuals (641 blood donors). All donors screened had anti-bacteroides and anti-enterobacterial LPS IgG present in their serum, with levels highly variable between different individuals. The degree of cross-reactivity of the serum IgG was examined by inhibition ELISA. This showed some cross-reactivity between the different anti-bacteroides LPS IgG, but very little between the anti-bacteroides LPS IgG and the anti-enterobacterial LPS IgG. Serum IgG was then measured daily over five to nine day periods in 12 sepsis patients (six survivors, six non-survivors) and in a healthy individual. In all patients IgG levels fluctuated to a greater extent than in a healthy subject. Variations all followed similar trends, and indicated that exposure to bacteroides LPS had occurred. In summary, this study demonstrated that there were some trends in antibody kinetics recognised which suggest that bacteroides LPS may be significant in sepsis.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available