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Title: Genetic and environmental factors associated with Helicobacter pylori and peptic ulcer disease
Author: Alkout, Abdulhamid M.
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1997
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The aims of the study were 1) to assess blood group and secretor status of local patients with peptic ulcer disease; 2) to determine if H. pylori binds to blood group antigens and if environmental factors such as fasting or smoking affect the binding; 3) to isolate bacterial adhesins that bind to the blood group antigens; 4) to determine if there are differences in the immune or inflammatory responses to H. pylori associated with ABO blood group or secretor status. There was a higher proportion of group O and non-secretors in the local patient group. In studies in which flow cytometry was used to assess bacterial binding to buccal epithelial cells or the gastric adenocarcinoma cell line (Kato III), binding of H. pylori was inhibited by pretreatment of the cells with monoclonal antibodies against Lewisa, Lewisb and H type 2 (the antigen of blood group O). Binding indices of H. pylori to buccal epithelial cells correlated with binding induces for H type 2 and Leb but not with Lea, suggesting the terminal fucose moieties of H type 2 and Lewis b are important epitopes for bacterial binding. Binding of H. pylori to buccal epithelial cells from smokers was significantly higher than to cells from non-smokers among patients referred to the gastroscopy clinic. In other experiments with cells from health volunteers, binding of H. pylori to cells from smokers was higher than to cells of non-smokers but the differences were not significant. Because each patient had fasted for approximately 12 hours before the cells were collected at the clinic, the effect of fasting and smoking were examined with buccal cells from1 5 pairs of smokers when fasting during Ramadan and after the fast when the donors were eating and drinking normally. In this group, binding of H. pylori and expression of H type 2 were significantly lower among the smokers.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available